Liquid nitrogen cryo-impacting: a new concept for cell disruption.
نویسندگان
چکیده
High-efficiency disruption of bacteria can be accomplished in 2 or more min by the new procedure of liquid nitrogen cryo-impacting. Release of the dipicolinic acid-Ca2+ chelate paralleled the breakage of Bacillus megaterium endospores. Lactate dehydrogenase activity was much better in supernates from liquid nitrogen cryo-impacting-broken Escherichia coli cells than in those from sonically treated and broken E. coli cells.
منابع مشابه
Defects and Structural Analysis of Multi-Wall Carbon Nano Tubes via Ball milling and Cryo-milling
Nano fillers are part of new studies to enhance various properties of fluids and solids. For example, adding nanoparticles as an enforcement in nanocomposites or as an additive to improve thermal or electrical properties in nano-fluids are of extreme importance in science and industry. There are numerous methods to uniformly disperse nanoparticles in fluid and solid phase. One of the well-used ...
متن کاملP-30: Developmental Capacity and Blastocyst Formation of Thawed Tetrahedral Versus Non-Tetrahedral 4-cell Stage Mouse Embryos After Vitrification
Background: It was reported in a literature that approximately one third of the 4-cell stage embryos did not exhibit a tetrahedral shape. Non-tetrahedral embryos showed a lower in vitro developmental potential than tetrahedral embryos. Recently vitrification technology has been widely employed for embryo cryopreservation. The objective of this study was to prove our hypothesis that vitrified - ...
متن کاملSuper-Resolution Microscopy Using Standard Fluorescent Proteins in Intact Cells under Cryo-Conditions
We introduce a super-resolution technique for fluorescence cryo-microscopy based on photoswitching of standard genetically encoded fluorescent marker proteins in intact mammalian cells at low temperature (81 K). Given the limit imposed by the lack of cryo-immersion objectives, current applications of fluorescence cryo-microscopy to biological specimens achieve resolutions between 400-500 nm onl...
متن کاملA Cryo-cooling Microfluidic Channel Device for Magnetic Resonance (mr) Microscopy System
ABSTRACT The development of a cryo-cooling microfluidic device for reducing the resistive noise of a magnetic resonance (MR) microcoil to improve its signal-to-noise ratio (SNR) in MR microscopy applications is presented. The radiofrequency (RF) spiral coil was cryo-cooled to liquid nitrogen temperature (-196oC) by the integrated microfluidic channels carrying liquid nitrogen for decreasing the...
متن کاملA magnetic resonance (MR) microscopy system using a microfluidically cryo-cooled planar coil.
We present the development of a microfluidically cryo-cooled planar coil for magnetic resonance (MR) microscopy. Cryogenically cooling radiofrequency (RF) coils for magnetic resonance imaging (MRI) can improve the signal to noise ratio (SNR) of the experiment. Conventional cryostats typically use a vacuum gap to keep samples to be imaged, especially biological samples, at or near room temperatu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied microbiology
دوره 30 3 شماره
صفحات -
تاریخ انتشار 1975