Liquid nitrogen cryo-impacting: a new concept for cell disruption.

نویسندگان

  • R A Smucker
  • R M Pfister
چکیده

High-efficiency disruption of bacteria can be accomplished in 2 or more min by the new procedure of liquid nitrogen cryo-impacting. Release of the dipicolinic acid-Ca2+ chelate paralleled the breakage of Bacillus megaterium endospores. Lactate dehydrogenase activity was much better in supernates from liquid nitrogen cryo-impacting-broken Escherichia coli cells than in those from sonically treated and broken E. coli cells.

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عنوان ژورنال:
  • Applied microbiology

دوره 30 3  شماره 

صفحات  -

تاریخ انتشار 1975